Comparative Advantage of the Novel Loop-Mediated Isothermal Amplification Technique over the Conventional Polymerase Chain Reaction

  • Umar Faruk Abdullahi Faculty of Medicine, Universiti Sultan Zainal Abidin, Kuala Terengganu, Terengganu, Malaysia
  • Ephraim Igwenagu University of Maiduguri, Maiduguri, Nigeria
  • Mimie Noratiqah Jumli Faculty of Health Science, Universiti Sultan Zainal Abidin, Kuala Terengganu, Terengganu, Malaysia
Keywords: Amplification, Diagnosis, DNA polymerase, Gene, Molecular diagnostic

Abstract

The role of gene amplification technology in the area of diagnostic medicine and other aspects of molecular biology has been very laudable. Molecular diagnostic has demystified the gray areas in disease diagnosis, making the process of disease management much easier and efficient. Several advancements in molecular diagnostics have been largely due to the introduction of the polymerase chain reaction (PCR). Hence, there is no doubt about the rank of the PCR, as one of the greatest molecular biological tools that existed in the last few decades. However, in this article we highlight the comparative advantages of a novel gene amplification technique called loop-mediated isothermal amplification (LAMP) over the conventional PCR. This innovative gene amplification technique has provided a solutions to the odds and limitations of the PCR through the unique features of the technique. The most interesting aspect of the LAMP, which is in contrast to the PCR is the affordability of the tool, hence, gives hope to the poor and resource-limited settings of the society. Other unique features of this novel technique are highlighted in this article.

Author Biographies

Umar Faruk Abdullahi, Faculty of Medicine, Universiti Sultan Zainal Abidin, Kuala Terengganu, Terengganu, Malaysia

Post Graduate Student, Department of Post Graduate

Ephraim Igwenagu, University of Maiduguri, Maiduguri, Nigeria

Lecturer, Department of Veterinary Pathology

Mimie Noratiqah Jumli, Faculty of Health Science, Universiti Sultan Zainal Abidin, Kuala Terengganu, Terengganu, Malaysia

Post Graduate Student, Department of Deitetic

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Published
2021-10-10
Section
Original Articles