Value of Argyrophilic Nucleolar Organizer regions in Benign, Premalignant, and Malignant Lesions of Cervix Uteri

  • Rajesh Mahobia Demonstrator, Department of Pathology
  • Muktesh Khandare Assistant Professor, Department of Pathology
  • Rashmi Nayak Assistant Professor, Department of Pathology
  • Bhagwan Singh Yadav Assistant Professor, Department of Pathology
Keywords: AgNORs staining technique, AgNORs counting procedure, Benign and Malignant lesions of cervix, Proliferative index


Objectives: The objective of this study is to evaluate the correlation of argyrophilic nucleolar organizer region (AgNOR) count between benign, premalignant cervical intraepithelial neoplasia (CIN), and malignant lesions of cervix.
Method: A retrospective study of 150 cases of paraffin-embedded blocks were histologically grouped as (A): Normal cervix, (B): Benign lesions - chronic cervicitis without dysplastic change, (C): Cervix with CIN I and II, (D): CIN III (E): Squamous cell carcinoma - well-differentiated, (F): Squamous cell carcinoma - moderately differentiated, (G): Squamous cell carcinoma - poorly differentiated (small cell non-keratinizing), and (H): Adenocarcinoma of cervix. The paraffin blocks were further subjected to thin sections, and silver staining (AgNOR) was done in the dark at room temperature. The AgNOR counting was done under oil immersion (×100). The number of black dots per 100 cells was counted and averaged.
Result: In this study, the mean AgNOR count was found to be statistically significant (t = 3.5 –21.8) at a confidence limit <0.0, clearly proving proliferative activity of the benign, premalignant, and malignant nucleoli.
Conclusion: AgNOR counting progressively increases directly in proportion to increased proliferative activity of the cells: (A+B) normal cervix and chronic cervicitis without dysplasia mean AgNOR count 3.5, (C): Cervix with CIN I and II mean AgNOR count 6.9, (D+E): CIN III and squamous cell carcinoma - well-differentiated mean AgNOR count 10.3, (F+G): Squamous cell carcinoma - moderately and poorly differentiated mean AgNOR count 16.16, and (H): Adenocarcinoma of cervix mean AgNOR count 21.8

Author Biographies

Rajesh Mahobia, Demonstrator, Department of Pathology

Netaji Subhash Chandra Bose Medical College Jabalpur, Madhya Pradesh, India

Muktesh Khandare, Assistant Professor, Department of Pathology

Netaji Subhash Chandra Bose Medical College Jabalpur, Madhya Pradesh, India

Rashmi Nayak, Assistant Professor, Department of Pathology

Netaji Subhash Chandra Bose Medical College Jabalpur, Madhya Pradesh, India

Bhagwan Singh Yadav, Assistant Professor, Department of Pathology

Netaji Subhash Chandra Bose Medical College Jabalpur, Madhya Pradesh, India


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