Comparison of Three Different Tests for Diagnosis of Enteric Fever

  • Mallika Sengupta Assistant Professor, Department of Microbiology
  • Manideepa Sengupta Professor, Department of Microbiology
Keywords: Blood culture, Enzyme-linked immunosorbent assay, Immunochromatography, Salmonella, Widal


Background: Enteric fever is a systemic illness caused by Salmonella Typhi and Salmonella Paratyphi. The different methods for diagnosis of enteric fever are blood, bone marrow, rarely stool and urine culture, nucleic acid detection, antibody detection by Widal test, and other rapid diagnostic tests.
Aim: The study was performed to evaluate the performance of tube Widal test, Typhiwell enzyme-linked immunosorbent assay (ELISA) test, and Typhifast, an immunochromatographic (ICT) test.
Materials and Methods: This study was carried out in the Department of Microbiology in a tertiary care center for 1 year (January–December 2015). The serum samples were collected from the patients with fever who had positive blood culture report. A total of 50 samples were included, of which 21 were positive for S. Typhi, 9 were positive for S. Paratyphi A, and 20 samples were positive for other organisms such as Escherichia coli (8 isolate), Klebsiella pneumoniae (8 isolate), and Staphylococcus aureus (4 isolate) by blood culture. The serum samples were used for doing the various tests for diagnosis of enteric fever such as tube Widal test, Typhiwell, ELISA test, and Typhifast, an ICT test.
Results: The three serological tests were performed and compared with blood culture, and it was found that Typhifast had a sensitivity of 70% and specificity of 100%, Typhiwell had a sensitivity of 90% and specificity of 75%, and Widal test had a sensitivity of 83.3% and specificity of 80%.
Conclusion: Widal test had a fairly good sensitivity and specificity, whereas Typhifast had a very good specificity but a lower sensitivity.

Author Biographies

Mallika Sengupta, Assistant Professor, Department of Microbiology

KPC Medical College and Hospital, Kolkata, West Bengal, India

Manideepa Sengupta, Professor, Department of Microbiology

Medical College and Hospital, Kolkata, West Bengal, India


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