An Antimicrobial Activity Assessment of Three Endodontic Sealers on Enterococcus faecalis, Candida albicans, and Staphylococcus aureus by a Direct Contact Test: An In Vitro Study

  • Trishnika Chakraborty (ORCID - 0000-0002-1389-2437) I.T.S Dental College and Research, Ghaziabad, Uttar Pradesh, India
  • Sonali Taneja I.T.S Dental College and Research, Ghaziabad, Uttar Pradesh, India
  • Shubhra Malik I.T.S Dental College and Research, Ghaziabad, Uttar Pradesh,India
Keywords: andida albicans, Direct contact test, Endodontic sealers, Enterococcus faecalis, Staphylococcus aureus

Abstract

Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH PlusAbstract
Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aim
of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida
albicans, and Staphylococcus aureus by direct contact test (DCT).
Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)
Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC
25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension
was drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were taken
at 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using one
way ANOVA and Tukey HSD test.
Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the time
intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum
for Group 1 (AH Plus) for both the time intervals (1 h and 24 h).
Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the
time intervals, followed by MTA Fillapex and AH Plus

Author Biographies

Trishnika Chakraborty (ORCID - 0000-0002-1389-2437), I.T.S Dental College and Research, Ghaziabad, Uttar Pradesh, India

Department of Conservative Dentistry and Endodontics.

Sonali Taneja, I.T.S Dental College and Research, Ghaziabad, Uttar Pradesh, India

MDS and Head, Department of Conservative Dentistry and Endodontics

Shubhra Malik, I.T.S Dental College and Research, Ghaziabad, Uttar Pradesh,India

MDS and Reader, Department of Conservative Dentistry and Endodontics

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Published
2021-09-28